PA-1（人卵巢畸胎瘤細胞）
CBP60800
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| I. General information | |
| Synonyms: | PA-1 |
| Background: | The line was established from cells taken from ascitic fluid. |
| Species: | Homo sapiens, human |
| Tissue: | ovary; derived from metastatic site: ascites |
| Disease: | teratocarcinoma |
| Gender: | female, 12 years, Caucasian |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin: X CSF1PO: 9,12 D13S317: 9,10 D16S539: 9,12 D5S818: 11 D7S820: 9 THO1: 7,9 TPOX: 11 vWA: 15,17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | HLA A28, B12 |
| Oncogene: | N-ras + (activated) |
| Tumor Formation: | Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
| Comments: | The cells form tightly knit colonies, and differentiate to form embryoid bodies when cultured in low serum concentration, or at low plating densities or when treated with 5-bromo-2'-deoxyuridine. The embryonic antigen PCC4 is expressed, but F9 is not detectable. For more information, please contact Cobioer (4008-750-250). |
