DR4 MEF(小鼠胚胎成纖維細胞)
CBP61134
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I. General information | |
Synonyms: | DR4 MEF |
Background: | The cell line was established by ATCC in 2003 from embryonic day 14 (E14) DR4 mouse embryos obtained from The Jackson Laboratory (Stock# 003208). Mouse embryo fibroblasts (MEFs) prepared from the DR4 mouse strain are resistant to neomycin, hygromycin, puromycin, and 6-thioguanine. Dr. Rudolf Jaenisch at the Massachusetts Institute of Technology developed the DR4 mouse strain by the intercrossing of three different strains: one bearing resistance genes neoR and puroR, a second bearing the resistance gene hygR, and a third bearing a natural deletion encompassing the Hprt gene. A series of matings incorporated all 4 drug resistance genes into the strain (Tucker et al., 1997; PubMed: 9278500). The background of the original DR4 strain was a mix of 129S4/SvJae, 129P2/OlaHsd, BALB/c, and C57BL/6. |
Species: | Mus musculus, mouse |
Tissue: | Embryo |
Disease: | malignant histiocytosis |
Gender: | Male and female mixed, 14 days gestation embryo |
Morphology: | fibroblast |
Growth Mode: | adherent |
Doubling Time: | N/A |
DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
Culture Medium: |
DMEM+15%FBS DR4 MEF完全培養(yǎng)基,# CBP61134M |
Cryopreservation medium: | 90%FBS+10%DMSO |
Antigen Expression: | N/A |
Receptor Expression: | N/A |
Oncogene: | N/A |
Genes Expressed: | N/A |
Cellular Products: | N/A |
Tumor Formation: | N/A |
Comments: | The growth of these cells should be arrested before being used as a feeder layer. ATCC has successfully irradiated and treated the cells with Mitomycin C for use as a feeder layer. If the MEFs are being used as a feeder layer for ES cells, it is not recommended to use them past passage no. 7 (P7). ATCC tested that this cell line is resistant to: G 418 (neomycin): 200 microgm/mL Puromycin: 0.4 microgm/mL Hygromycin: 110 microgm/mL 6-Thioguanine: 2.5 microgm/mL For more information, please contact Cobioer (4008-750-250). |