M-07e（人巨細(xì)胞白血病細(xì)胞）
CBP60791
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| I. General information | |
| Synonyms: | M-07e |
| Background: | established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-ß, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells |
| Species: | Homo sapiens, human |
| Tissue: | operipheral blod |
| Disease: | acute megakaryoblastic leukemia |
| Gender: | famale,6-month-old |
| Morphology: | acute megakaryoblastic leukemia |
| Growth Mode: | single round cells in suspension, a few cells are lightly adheren |
| Doubling Time: | 40 hours |
| Culture Medium: |
80% RPMI-1640 + 20% FBS + GM-CSF (10 ng/ml) or 10% vol conditioned medium of cell line 5637 M-07e完全培養(yǎng)基，# CBP60791M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - Immunology: CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR Cytogenetics: human near-diploid karyotype - 46(45-46)<2n>XX, t(11;21)(p11;p13), add(13)(p13), add(22)(p13) For more information, please contact Cobioer (4008-750-250). |
