ACHN(人腎腺癌細(xì)胞)
CBP60704
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產(chǎn)品描述
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I. General information | |
Synonyms: | ACHN |
Background: | The ACHN cell line was initiated in November, 1979 from the malignant pleural effusion of a 22-year-old Caucasian male with widely metastatic renal adenocarcinoma (autopsy confirmed). Cells were seeded directly to culture flasks in Eagle's MEM with 10% FBS, then maintained and passaged 150 days in flasks. Cells were then inoculated subcutaneously into nude mice. After 4 weeks, palpable, locally invasive tumors were noted. Both the original cells (ACHN) and those recovered from nude mouse tumors were growth-inhibited by human interferons. |
Species: | Homo sapiens, human |
Tissue: | kidney; derived from metastatic site: pleural effusion |
Disease: | renal cell adenocarcinoma |
Gender: | Caucasian,male,22 years |
Morphology: | epithelial |
Growth Mode: | adherent |
DNA Profile: | Amelogenin: X CSF1PO: 11 D13S317: 12 D16S539: 12,13 D5S818: 12 D7S820: 9,11 THO1: 8 TPOX: 8,11 vWA: 16,17 Cobioer’s Cell Line Authentication Service |
Culture Medium: |
MEM+10%FBS+1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) ACHN完全培養(yǎng)基,# CBP60704M |
Cryopreservation medium: | 90%FBS+10%DMSO |
Oncogene: | p53 +; pRB + |
Genes Expressed: | p53 +; pRB + |
Tumor Formation: | Yes, in nude mice,(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
Comments: | Growth is inhibited by human interferon [Hogan, T. F., personal communication]. For more information, please contact Cobioer (4008-750-250). |