SRE.L-Luc/HEK293
CBP74201
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I. Background | |
The SRE Reporter – HEK293 cell line is designed for monitoring the activity of the MAPK/ERK signaling pathway. The SRE Reporter – HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line. | |
II. Introduction | |
Host Cell: | HEK293 |
Expressed gene: | SRE.L-Luciferase |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Synonym(s): | SRE cell line, MAPK reporter, ERK reporter |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | DMEM+10%FBS+100ug/ml hygromycin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | Functional(Report Gene) Assay |
III. Description of Host Cell Line | |
Organism: | Homo sapiens, human |
Tissue: | Embryonic kidney |
Disease: | Normal |
Morphology: | Epitheloid cell |
Growth Properties: | Adherent |
IV. Representative Data |
Figure 1.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer).