GLP1R/CRE-Luc/BHK
CBP71405
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| I. Background | |
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GLP-1R 與其他四種胰高血糖素受體（GCGR、GLP-2R、GIPR 和 GHRHR）一起屬于 促胰液素 (B1 類) GPCR 家族，其內(nèi)源性配體是肽激素。 B1 類 GPCRs 在 N 端具有一個較 大的、結(jié)構(gòu)保守的胞外結(jié)構(gòu)域（ECD），由 120-160 個殘基組成，形成三層 α-β-β-α 折疊， 并由三個層間二硫鍵穩(wěn)定。 |
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| II. Description | |
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GLP1R CRE-Luc BHK 報告基因藥靶模型很好的模擬了體內(nèi) GLP1R 的信號轉(zhuǎn)導(dǎo)過程， 原理見下圖所示。
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| III. Introduction | |
| Host Cell: | BHK |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | GLP1R |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM+10% FBS+1% Non Essential Amino Acids (NEAA)+1 mM sodium pyruvate+2ug/ml puromycin+800ug/ml hygromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for GLP1R |
| Transducer: | Gs |
| IV. Description of Host Cell Line | |
| Organism: | Mesocricetus auratus, hamster, Syrian golden |
| Tissue: | kidney |
| Disease: | Normal |
| Morphology: | fibroblast |
| Growth Properties: | Adherent |
| V.Representative Data | |
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Figure 2.Dose response of GLP-1(1-37) in GLP1R CRE-Luc BHK Cell(C22). |
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