hMSLN/MC-38
CBPG0007
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產(chǎn)品描述
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I. Background | |||
間皮素(Mesothelin)又被稱為 MSLN,它是由 MSLN 基因編碼的一種細(xì)胞表面糖蛋 白。MSLN 基因編碼一種前體蛋白,經(jīng)蛋白水解處理生成兩種蛋白產(chǎn)物,主要位于正常間 皮細(xì)胞表面,兩種蛋白產(chǎn)物為巨核細(xì)胞增強(qiáng)因子(megakaryocyte potentiating,MPF)和間 皮素(Mesothelin,MSLN)。間皮素(MSLN)可黏附于細(xì)胞膜上,主要表達(dá)于胸腹膜與 臟器的鏈接面上,黏附于間皮細(xì)胞表面,在其余正常組織重表達(dá)量低。MSLN 在許多實(shí)體 瘤中呈現(xiàn)高表達(dá),最常見于間皮瘤、上皮性卵巢癌和胰腺癌,但也見于肺和子宮惡性腫瘤 以及膽管癌。間皮素在卵巢癌和間皮瘤中的表達(dá)最高,較高的表達(dá)與晚期腫瘤分期和較高的轉(zhuǎn)移率相關(guān)。 | |||
II. Introduction | |||
Host Cell: | MC-38 | ||
Expressed gene: | hMSLN | ||
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
Freeze Medium: | 90% FBS+10% DMSO | ||
Culture Medium: | DMEM+10%FBS+2 μg/ml puromycin | ||
Mycoplasma Testing: | Negative | ||
Storage: | Liquid nitrogen | ||
III. Description of Host Cell Line | |||
Organism: | Mus musculus, mouse | ||
Tissue: | Colon | ||
Morphology: | Epitheloid cell | ||
Growth Properties: | Adherent | ||
IV. Representative Data | |||
Figure 1. Recombinant hMSLN/MC38 stably expressing MSLN. |
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